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Volume: 7, Issue: 1, Jan-Feb, 2019
DOI: 10.7324/JABB.2019.70104

Research Article

Effect of Estradiol-17ß on Embryonic Tolerance, Growth, and Muscular compactness of Giant Freshwater Prawn, Macrobrachium rosenbergii


Noppakun Pakdeenarong

  Author Affiliations


Abstract

Effects of estradiol-17β on the giant freshwater prawn were observed in case of the embryonic tolerance, growth, development, survival rate, yolk adsorption, eye appearance, and muscular compactness. The methods were designed in two steps; those were the tolerance and the growth. In the two hours after spawning, embryos were immersed in 10, 50, 100, or 150 µg/ml estradiol-17β solution for two days, and the controls were treated with 15% artificial seawater. The tolerance was monitored in a separate experiment; they were immersed in 10, 50, 100, or 150 µg/ml estradiol-17β solutions showed that the mortality rate at 150 µg/ml estradiol-17β was 31.66%. The growth represented by yolk adsorption, hatching rate, and eye appearance. The results showed that the eyes appeared earlier than those of the controls. The survival rates were investigated in other experiments. The results showed that the highest survival rate was 48.16%, observed in the 50 µg/ml estradiol-17β treatment. Therefore, estradiol might accelerate the growth as indicated by the number of days for eye appearance being shorter period than in the control treatment. The eyes of the embryos treated with 50, 100, and 150 µg/ml estradiol-17β appeared on day 10, whereas those in the control were observed on day 16. Hatching rate was tending to high in 150 µg/ml estradiol-17β but those were not significance with the control. Yolk adsorption was found in treated embryo rather than those of the controls. The pattern of yolk cluster distribution was differing from the control. The muscle tissue was observed on day 20 after the histological process. The results showed that the bundles muscle cells were more compactness and were larger, denser, and stronger with increasing concentrations of estradiol-17β than that the controls. Therefore, estradiol-17β should be applied to stimulate growth and might be introduced with the feed to the prawn industry and manufacturing

Keywords:

Estradiol-17ß, Embryonic development, Muscles, Giant freshwater prawn.



Citation: Pakdeenarong N. Effect of Estradiol-17\ß on Embryonic Tolerance, Growth, and Muscular compactness of Giant Freshwater Prawn, Macrobrachium rosenbergii. J App Biol Biotech. 2018. 2019;7(01):016-020. DOI: 10.7324/JABB.2019.70104


Copyright: Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited.

References

1. Van-Wormhoudt A, Bellon-Humbert C. Crustacean farming: The biological basis. In: Bernabe G, editor. Aquaculture Biology and ecology of cultured species. New York: Ellis Horwood; 1994, p. 179-201.

2. New MB, Valenti WC. Freshwater prawn culture: The Farming of Macrobrachium rosenbergii. Oxford: Blackwell Science; 2000. https://doi.org/10.1002/9780470999554

and progesterone in the reproducing females of the mud crab Scylla serrata. Comparative Biochemistry and Physiology Part A . 2001; 130: 283-294.b3. Warrier, SR, Tirumalai R, Subramoniam T. Occurrence of vertebrate steroids, estradiol-17 https://doi.org/10.1016/S1095-6433(01)00385-3

4. Anderson HR, Halling-Sorensen B, Kusk KO. A parameter for detecting estrogenic exposure in the copepod Acartia tonsa. Ecotoxicology and Enviromental Safety. 1999 ; 41: 56-61. https://doi.org/10.1006/eesa.1999.1800

5. De Loof A, Huybrechts R, Kotanen S. Reproduction and love: stragies of the organism's cellular defense system. Comparative Biochemistry and Physiology Part C. 1998; 120: 167-176. https://doi.org/10.1016/S0742-8413(98)10007-5

concentration in tissue of the scleractinian coral, Montipora verrucosa. Comparative Biochemistry and Physiology Part A. 1999; 122: 85-92.b6. Tarrant AM, Atkinson S, Atkinson MJ. Estrone and estradiol-17 https://doi.org/10.1016/S1095-6433(98)10155-1

7. Barker MF, Xu RA. Effects of estrogens on gametogenesis and steroid levels in the ovaries and pyloric caeca of Scleasterias mollis (Echinodermata:Asteroidea). Invertebrate Reproduction and Development. 1993.; 24: 53-58. https://doi.org/10.1080/07924259.1993.9672331

8. Coveney D, Shaw G, Renfree MB. Estrogen-induced gonadal sex reversal in the Tammar wallaby. Biol. Reprod. 2001; 65: 613-621. https://doi.org/10.1095/biolreprod65.2.613

9. Swartz WJ. Effect of steroids on definitive localization of primordial germ cells in the chick embryo. Journal of Anatomy. 1975; 142: 499-513. https://doi.org/10.1002/aja.1001420407

in male flounders (Platicthys flesus). Comparative Biochemistry and Physiology Part B, 1979; 63: 1-6.b10. Emmerson J, Korsgaard B, Peterson I. Dose response kinetics of serum vitellogenin, liver DNA, RNA, protein and lipid after induction by estradiol-17 https://doi.org/10.1016/0305-0491(79)90225-6

11. Christiansen T, Korsgaard B, Jesperson, A. Effects of nonylphenol and 17\ß-estradiol on vitellogenin synthesis, testicular structure and cytology in male eelout Zoarces viviparous. Journal of Experimental Biology, 1998; 201: 179-192.

12. Tata JR. Induction and regulation of vitellogenin synthesis by estrogen. In: Litwak G., editor. Biochemical actions of hormones. Vol 5. New York : Academic Press; 1978. https://doi.org/10.1016/B978-0-12-452805-5.50016-8

13. Quinitio ET, Yamauchi K, Hara A, Fuji A. Profiles of progesterone and estradiol-like substances in the hemolymph of female Pandalus kessleri during annual reproductive cycle. General and Comparative Endocrinology, 1991; 81: 343-348. https://doi.org/10.1016/0016-6480(91)90160-8

-reductase inhibitors, antiandrogen, and sex steroids on Bidder's organs development and gonadal differentiation in Bufo bufo tadpoles. Journal of Experimental Zoology, 1998; 280: 245-259.a14. Petrini S, Zaccanti, F. The effects of aromatase and 5 https://doi.org/10.1002/(SICI)1097-010X(19980215)280:3< 245::AID-JEZ6>3.0.CO;2-N

15. Ghosh D, Ray AK. Estrogen stimulated lipogenic activity in the ovary of the freshwater prawn, Macrobrachium rosenbergii. Invertebrate Reproduction and Development, 1994; 25: 43-47. https://doi.org/10.1080/07924259.1994.9672367

16. Damrongphol P, Jaroensastraraks P. Morphology and regional distribution of the primordial germ cells in the giant freshwater prawn Macrobrachium rosenbergii. ScienceAsia, 2001; 27: 15-19. https://doi.org/10.2306/scienceasia1513-1874.2001.27.015

17. Pakdeenarong N, Damrongphol P. Effects of estradiol-17 on embryos and larvae of the giant freshwater prawn, Macrobrachium rosenbergii (Decapoda, Palaemonidae) Crustaceana, 2006;79: 563-572. https://doi.org/10.1163/156854006777584278

18. Ghosh D, Ray AK. 17\ß-hydroxysteroid dehydrogenase activity in the hepatopancreas and ovary of freshwater prawn, Macrobrachium rosenbergii: Relation to ovarian condition and estrogen treatment. General Comparative. Endocrinoogy, 1993; 89: 248-254. https://doi.org/10.1006/gcen.1993.1030

19. van den Beld. AW, de Jong FH, Grobbee DE, Pols HAP, Lamberts SWJ. Measures of Bioavailable Serum Testosterone and Estradiol and Their Relationships with Muscle Strength, Bone Density, and Body Composition in Elderly Men. Journal of Clinical Endocrinology & Metabolism, 2000; 85: 3276-3282.

20. Enns DL, Tiidus PM. The Influence of Estrogen on Skeletal Muscle. Sports Medicine, 2012; 40: 41-58. https://doi.org/10.2165/11319760-000000000-00000

21. Lee FY, Chang CF. Hepatopancreas is the likely organ of vitellogenin synthesis in the freshwater prawn, Macrobrachium rosenbergii. Journal of Experimental Zoology, 1999; 248: 798-806. https://doi.org/10.1002/(SICI)1097-010X(19991201)284:7< 798::AID-JEZ10>3.0.CO;2-C

22. Ghosh , Ray AK. Subcellular Action of Estradiol-17\ß in a Freshwater Prawn, Macrobrachium rosenbergii. General Comparative Endocrinology, 1993; 90: 274-281 https://doi.org/10.1006/gcen.1993.1082

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