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Volume: 2, Issue: 2, March-April, 2014
Changes in chromatin active fraction of germinating seedlings of wheat under the effect of EMI EHF
P.O. Vardevanyan, M.A. Parsadanyan, M.A. Shahinyan, M.R. Darbinyan
The effect of electromagnetic irradiation with extremely high frequencies (EMI EHF) on chromatin active fraction of wheat germinating seedlings has been studied. In present work the first zone of saccharine gradient was investigated which is enriched with transcribing genes and comprises higher quantity of AT-sequences compared with other zones. It was shown that the effect of EMI EHF results in chromatin activation. At germination and EMI EHF effect on the differential melting curves besides the pronounced high temperature peak which is corresponded to the melting of stabilized DNA in non active chromatin content, gradually the second low temperature peak is appeared which in its turn is corresponded to the melting of destabilized DNA in active chromatin content.
Keywords: Area under the integral curve, chromatin active fraction, differential melting curve, EMI EHF, wheat dry seeds and germinating seedlings.