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Volume: 6, Issue: 4, July-August, 2018
DOI: 10.7324/JABB.2018.60407

Research Article

Hepatoprotective activity of methanolic shoot extract of Bambusa bambos against carbon tetrachloride induce acute liver toxicity in Wistar rats

A. A. Shetti Suman Patil, H Joy Hoskeri, P Rajeev, Bhushan Kulkarni, Geetanjali R Kamble, Gurusiddhesh B Hiremath, Vishal Kalebar, S V Hiremath

  Author Affiliations


Abstract

The young shoots of Bambusa bambos are used ayurvedic medicines in India. Young shoots content various chemical components such as cholin, betain, urease, cyanogenetic glucosides, oxalic acid, and benzoic acid. It has antiulcer, antifertility, anti-inflammatory, and antioxidant functions. In the present study, the hepatoprotective activity of methanolic shoot extract of B. bambos was tested against carbon tetrachloride (CCl4)-induced hepatotoxicity in rats. Female Wistar rats were divided into five groups; Group I served as normal control. Groups II-VI were administered CCl4 mixed with olive oil 1:1 intraperitoneally (1 mL/kg body weight), after every 72 h for 16 days. Group II was CCl4 negative control. Groups IV and V received methanolic shoot extract mixed with olive oil, 200 mg and 400 mg/ kg body weight, respectively. Group III received silymarin 50 mg/kg body weight for 16 days orally once daily. Methanolic extract attenuated the increase in aspartate amino transaminase (AST) and alanine amino transaminase (ALT) as well as alkaline phosphatase (ALP) and total bilirubin that occur during liver injury after CCl4 injection. Outcome of the present study suggests that treatment with methanolic shoot extract of B. bambos- induced reduction in ALT, AST, ALP, and total bilirubin in rats indicating hepatoprotective potential of the extract.

Keywords:

Bambusa bambos, Hepatoprotective, Methanolic extract, Carbon tetrachloride.



Citation: Patil S, Shetti A, Hoskeri HJ, Rajeev P, Kulkarni B, Kamble GR, Hiremath GB, Kalebar V, Hiremath SV. Hepatoprotective activity of methanolic shoot extract of Bambusa bambos against carbon tetrachloride induce acute liver toxicity in Wistar rats. J App Biol Biotech. 2018;6(04):37-40. DOI: 10.7324/JABB.2018.60407


Copyright: Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited.

References

1. Sun F, Hamagawa E , Tsutsui C, Ono Y, Ogiri Y, Kojo S. Evaluation of oxidative stress during apoptosis and necrosis caused by carbon tetrachloride in rats liver. Biophys. Acta. 2001;1535: 186–191. https://doi.org/10.1016/S0925-4439(00)00098-3

2. Park EJ, Jeon CH, Ko G, Kim J, Sohn DH. Protective effect of curcumin in rat liver injury induced by carbon tetrachloride.J. Pharm. Pharmacol. 2000; 52: 437–440. https://doi.org/10.1211/0022357001774048

3. G. Venkat Raji Reddy, R.Vijay Kumar, V. Rama, M. Krishna Reddy and Y. Narsimha Reddy. Preliminary Hepatoprotective Activity of Medicinal Plant Extracts Against Carbon Tetrachloride Induced Hepatotoxicity In Albino Rats. International Journal of Recent Scientific Research.2015; Vol. 6(7): 4946-4951,

4. Slinkard K, Singleto, V L. Total phenol analyses automation and comparison with manual methods. Am. J. Enol. Viticult. 1977; 28: 49-55.

5. Khare CP. Indian Medicinal Plants. An Illustrated Dictionary. Springer publication. New Delhi, India. 2007

6. Rathod JD, Pathak NL, Patel RG, Jivani NP, Bhatt NM. Phytopharmacological Properties of Bambusa arundinacea as a potential medicinal tree: An overview. J Appl Pharm Sci 2011;1:27-31.

7. Siti Syariah Mamat, Mohamad Fauzi Fahmi Kamarolzaman, Farhana Yahya, Nur Diyana Mahmood, Muhammad Syahmi Shahril, Krystal Feredoline Jakius, Norhafizah Mohtarrudin, Siew Mooi Ching, Deny Susanti, Muhammad Taher, Zainul Amiruddin Zakaria1. Methanol extract of Melastoma malabathricum leaves exerted antioxidant and liver protective activity in rats. Complementary and Alternative Medicine. 2013; 13:326-331. https://doi.org/10.1186/1472-6882-13-326

8. Lin Yuan, Xuezhu Gu, Zhenhua Yin,Wenyi Kang, Afr J Tradit. Antioxidantactivities In Vitro And Hepatoprotective Effects Of Nelumbo Nucifera Leaves In Vivo Complement Altern Med. 2014; 11(3):85-91

9. Akah PA, Odo CI. Hepatoprotective effect of the solvent fractions of the stem of Hoslundia opposita Vahl (Lamiaceae) against carbon tetrachloride- and paracetamol-induced liver damage in rats. Int J Green Pharm. 2010; 4:54–58. https://doi.org/10.4103/0973-8258.62159

10. Huo, Hai Zhong, Bing Wang, Yong Kang Liang, Yong Yang Bao, and Yan Gu. "Hepatoprotective and antioxidant effects of licorice extract against CCl4-induced oxidative damage in rats." International journal of molecular sciences . 2011 12 (10): 6529-6543. https://doi.org/10.3390/ijms12106529

11. Singh Suruchi, Maryam Bincy Thomas, Sharada Pal Singh, Bhowmik D.Plants used in hepatoprotective remedies in traditional Indian medicine. Ind. J. Res. Pharm. Biotechnol 2013;(1): 58-63.

12. Agarwal AK , Mehendale JK. Potentiation of carbon tetrachloride hepatotoxicity and lethality by chlordecone in female rats. Toxicology. 1983; 26: 231-242. https://doi.org/10.1016/0300-483X(83)90084-7

13. Gupta R K, Talib Hussain G, Panigrahi Avik Das, Gireesh Narayan Singh, K. Sweety, Md Faiyazuddin, Chandana Venkateswara Rao. Hepatoprotective effect of Solanum xanthocarpum fruit extract against CCl 4 induced acute liver toxicity in experimental animals. Asian Pacific journal of tropical medicine. 2011;4 (12): 964-968. https://doi.org/10.1016/S1995-7645(11)60227-7

14. Okokon JE, Simeon JO, Umoh EE. Hepatoprotective activity of the extract of Homalium letestui stem against paracetamol-induced liver injury. Avicenna Journal of Phytomedicine. 2017;7(1):27-36.

15. Dikshit Piyush, Mool Kumar Tyagi, Kirtikar Shukla, Sonal Sharma, Jasvindar Kaur Gambhir, Rimi Shukla. Hepatoprotective effect of stem of Musa sapientum Linn in rats intoxicated with carbon tetrachloride. Ann Hepatol 2011;10 (3): 333-339.

16. Putri Herwandhani, Standie Nagadi, Yonika Arum Larasati, Nindi Wulandari, Adam Hermawan. Cardioprotective and hepatoprotective effects of Citrus hystrix peels extract on rats model. Asian Pacific journal of tropical biomedicine. 2013; 3(5): 371-375. https://doi.org/10.1016/S2221-1691(13)60079-9

17. Akbartabar Toori M, Joodi B, Sadeghi H, et al. Hepatoprotective activity of aerial parts of Otostegia persica against carbon tetrachloride-induced liver damage in rats. Avicenna Journal of Phytomedicine. 2015;5(3):238-246.

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