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Volume: 6, Issue: 4, July-August, 2018
DOI: 10.7324/JABB.2018.60403

Research Article

Substrate optimization for cultivation of Pleurotus ostreatus on lignocellulosic wastes (coffee, sawdust, and sugarcane bagasse) in Mizan–Tepi University, Tepi Campus, Tepi Town

Dagnew Bitew Tarko, Abel Mandefro Sirna

  Author Affiliations


Tepi town is rich in lignocellulosic wastes such as coffee waste (Cw), sawdust (Sd), and sugarcane bagasse (Sb) which possibly used as mushroom substrate. Therefore, the purpose of this study was to assess the suitability of three locally available substrates (Cw, Sd, and Sb) and their combinations for the cultivation of Pleurotus ostreatus (Jacq. ex Fr.) P. Kummer . Spawn was prepared with sorghum supplemented with 5% wheat bran and 1% gypsum. Main substrates were weighed, soaked in water, and steam sterilized for 1 h in plastic bags and inoculated with fully grown spawn. All inoculated bags were arranged in completely randomized design on shelves in mushroom growing room at ambient temperature (23 ± 2°C). Following days taken for complete mycelial colonization, pinhead formation, fruit body formation and maturation, biological efficiency (BE), stipe length, and pileus diameter of mature mushroom were recorded and analyzed using SPSS version 16. Majority of substrate showed significant variation in days taken for mycelial colonization, pinhead formation, and BE at P ≤ 0.05, whereas incubation periods between flushes, stipe length and pileus diameter were not significantly varied. Despite lignocellulosic waste being a menace to the environment represent a potential bioresource for production of oyster mushrooms.


Biological efficiency, Lignocellulosic waste, Pleurotus ostreatus (Jacq. ex Fr.) P. Kummer, Spawn, Substrate optimization.

Citation: Bitew TD, Mandefro SA. Substrate optimization for cultivation of Pleurotus ostreatus on lignocellulosic wastes (coffee, sawdust, and sugarcane bagasse) in Mizan–Tepi University, Tepi Campus, Tepi Town. J App Biol Biotech. 2018;6(04):14-20. DOI: 10.7324/JABB.2018.60403

Copyright: Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited.


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