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Volume: 6, Issue: 4, July-August, 2018
DOI: 10.7324/JABB.2018.60402

Research Article

In vitro conservation and propagation of the endemic species of “floating hearts” (Nymphoides krishnakesara Joseph and Sivar. - Menyanthaceae) as a conservation strategy

Swetha Thilak T1, Anusree P1, Saranya Babu Jayaprakash CM1, Smitha RB1, Minoo Divakaran2, Madhusoodanan PV1, Prakashkmar R1

  Author Affiliations


Nymphoides krishnakesara Joseph and Sivar. (Menyanthaceae), an endangered aquatic angiosperm, endemic to Kerala, was multiplied and propagated through in vitro culture. It is a rare species with very restricted distribution occurring in a single location. It is an annual herb, grows in shallow temporary pools on laterite. The nodal explants when cultured in Murashige and Skoog (MS) medium attained bud break in 20 days. Maximum multiple shoot proliferation observed in 1.0 mg/L benzyl amino purine (80 shoots/explant). Shoots developed in vitro were rooted in MS medium with both indole 3-butyric acid (IBA) (1.0 mg/L) and naphthalene acetic acid (mg/L). Roots developed in IBA are found to be more favorable based on the histological studies. The rooted plantlets were then transferred to the field after hardening and they flowered after 2 months. Total time taken from explants to flowering is 10 months. This work standardizes an easy protocol for mass production of plantlets, and thus enhances conservation of this endemic and rare aquatic plant. The hardened plants were successfully reintroduced and recorded 100% survival.


Endemic, Nymphoides, In vitro, Multiplication, Protocol.

Citation: Thilak ST, Anusree P, Jayaprakash CMSB, Smitha RB, Divakaran M, Madhusoodanan PV, Prakashkumar R. In Vitro conservation and propagation of the endemic species of “floating hearts” (Nymphoides krishnakesara Joseph and Sivar. - Menyanthaceae) as a conservation strategy. J App Biol Biotech. 2018;6(04):9-13. DOI: 10.7324/JABB.2018.60402

Copyright: Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited.


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