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Volume: 6, Issue: 4, July-August, 2018
DOI: 10.7324/JABB.2018.60401

Research Article

Therapeutic potential of harmaline, a novel alkaloid, against cervical cancer cells in vitro: Apoptotic induction and DNA interaction study

Paromita Bhattacharjee, Sarita Sarkar, Tapas Ghosh, Kakali Bhadra

  Author Affiliations


Abstract

The study emphasizes the growth inhibitory effect of harmaline on HeLa (human cervical cancer) cell line and mode and mechanism of binding with CT DNA (calf thymus DNA). The results of cytotoxic study performed through MTT assay indicates that harmaline have concentration dependent growth inhibitory effect on HeLa cell line with GI50 value of 28 µM. Furthermore, the alkaloid induced DNA damage and changes in mitochondrial membrane potential in the cell line. The alkaloid shows reactive oxygen species dependent cellular damage with significant arrest in G2 /M population of the cell. Biophysical experiments further established the Ki w value (product of cooperative binding affinity and the cooperative factor) of harmaline with CT DNA to be 5.60 × 105 M−1. Harmaline showed a progressive quenching of the fluorescence emission spectra. Circular dichroism study shows significant structural changes of DNA with subsequent induction of optical activity in the bound achiral alkaloid molecule. The alkaloid stabilizes the DNA to 10°C. Intercalated state of harmaline inside DNA helix was shown by viscometric and ferrocyanide quenching. The results highlight the importance of alkaloid- DNA interaction for developing nucleic acid based therapeutic agents.

Keywords:

Beta carboline, Cytotoxicity, Reactive oxygen species, Spectroscopy, Isothermal calorimeter.



Citation: Bhattacharjee P, Sarkar S, Ghosh T, Bhadra K. Therapeutic potential of harmaline, a novel alkaloid, against cervical cancer cells in vitro: Apoptotic induction and DNA interaction study. J App Biol Biotech. 2018;6(04):1-8. DOI: 10.7324/JABB.2018.60401


Copyright: Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited.

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