Home >Archive

Volume: 6, Issue: 3, May-June, 2018
DOI: 10.7324/JABB.2018.60309

Research Article

Eco-friendly Industrial wastewater treatment: Potential of mesophilic bacterium, Pseudomonas putida (ATCC 49128) for hydrogen sulfide oxidation

Mani Malam Ahmad1 2, Abd. Aziz Mohd Azoddein1, Mohammed Saedi Jami3

  Author Affiliations


Abstract

The effectiveness and environmental friendliness of biological sulfide oxidation have endeared it to many researchers, mainly for its potential to offer the best alternative for the evacuation of various forms of sulfide. The present study was conducted to assess the potential of gammaproteobacteria, Pseudomonas putida (ATCC 49128), to biodegrade sulfide significantly in a suspended medium of batch reactor type. Sulfide oxidation efficiency was measured spectrophotometrically under defined operational conditions of temperature, agitation, aeration, and influent sulfide concentrations. Sulfide reduction rates were observed at three disproportionate sulfide concentrations of 100 ppm S2− L−1 d−1, 300 ppm S2− L−1 d−1, and 500 ppm S2− L−1 d−1. The simple statistical analysis was employed in the interpretation of the data and presented graphically. The results indicated that it was possible to realize sulfide removal efficiency of 45–70% within the first 6 h of start-up and 96–100% in 24 h period. On the other hand, the corresponding exponential cell growth recorded was 3.91, 3.80, and 3.61 in 100 ppm, 300 ppm, and 500 ppm, respectively. This also translates to cell biomass synthesis (cell dry weight) of 0.61 g/L, 0.58 g/L, and 0.50 g/L in 100, 500, and 300 ppm, respectively. In conclusion, it can be deduced that this inoculum can utilize different sulfide concentration for its growth and biosynthesis and thus can be employed to treat sulfide contaminated wastewater in a suspended growth form.

Keywords:

Eco-friendly, Treatment, Sulfide, Pseudomonas putida, Potential.



Citation: Ahmad MM, Azoddein AAM, Jami MS. Eco-friendly Industrial wastewater treatment: Potential of mesophilic bacterium, Pseudomonas putida (ATCC 49128) for hydrogen sulfide oxidation. J App Biol Biotech. 2018;6(3):53-57. DOI: 10.7324/JABB.2018.60309


Copyright: Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited.

References

1. Enning D, Garrelfs J. Corrosion of iron by sulfate-reducing bacteria: New views of an old problem. Appl Environ Microbiol. 2014;80(4):1226–36. https://doi.org/10.1128/AEM.02848-13

2. Zytoon MA-M, AlZahrani AA, Noweir MH, El-Marakby FA. Bioconversion of high concentrations of hydrogen sulfide to elemental sulfur in airlift bioreactor. Hindawi Publ Corp Sci World J. 2014;2014:675673. Available from: http://www.scopus.com/inward/record.url?eid=2-s2.0-84934934801&partnerID=tZOtx3y1

3. Kim JH, Rene ER, Park HS. Biological oxidation of hydrogen sulfide under steady and transient state conditions in an immobilized cell biofilter. Bioresour Technol. 2008;99(3):583–8. https://doi.org/10.1016/j.biortech.2006.12.028

4. Lorna Guerreroa, Silvio Montalvo Cesar Huilinir, Jose Luis Campos, Andrea Barahona RB. Advances in the biological removal of sulphides from aqueous phase in anaerobic processes : A review. Environ Rev. 2015;(November):1–61.

5. Nur Hafizah Azizana, Kasing Ak Apunb, Lesley Maurice Bilungb, Micky Vincentb, Hairul Azman Roslanb AASAAH. Crude Oil Bioremediation by Indigenous Bacteria Isolated from Oily Sludge. J Teknol. 2016;2:61–6.

6. Tang K, Baskaran V, Nemati M. Bacteria of the sulphur cycle: An overview of microbiology, biokinetics and their role in petroleum and mining industries. Biochem Eng J. 2009;44(1):73–94. https://doi.org/10.1016/j.bej.2008.12.011

7. Zhishu Liang, Taicheng An, Guiying Li ZZ. Aerobic biodegradation of odorous dimethyl disulfide in aqueous medium by isolated Bacillus cereus GIGAN2 and identification of transformation intermediates. Bioresour Technol. 2015;175:563–8. Available from: http://dx.doi.org/10.1016/j.biortech.2014.11.002 https://doi.org/10.1016/j.biortech.2014.11.002

8. Reshma JK& AM. Biodegradation of Phenol-Aerobic and Anaerobic Pathways. Int J Sci Nat. 2014;5(3):366–87.

9. Hamid Rashedi, Ali Izadi MEB. Optimization of Operational Parameters in Rhamnolipid Production by Pseudomonas aeruginosa MM1011 in a Miniaturized Shaken Bioreactor. J Appl Biotechnol Reports. 2015;2(3):271–8.

10. Wang SJ, Loh KC. Modeling the role of metabolic intermediates in kinetics of phenol biodegradation. Enzyme Microb Technol. 1999;25(3–5):177–84. https://doi.org/10.1016/S0141-0229(99)00060-5

11. Durve A, Chandra N. FT-IR analysis of bacterial biomass in response to heavy metal stress. Int Jouranl Biotechology. 2014;112:386–91.

12. Fajardo, C. Mosquera-Corral, A. Campos, J. L. Mendez R. Autotrophic denitrification with sulphide in a sequencing batch reactor. J Environ Manage. 2012;113:552–6. Available from: http://dx.doi.org/10.1016/j.jenvman.2012.03.018 https://doi.org/10.1016/j.jenvman.2012.03.018

13. Kleinjan WE, De Keizer A, Janssen AJH. Equilibrium of the reaction between dissolved sodium sulfide and biologically produced sulfur. Colloids Surfaces B Biointerfaces. 2005;43(3–4):228–37. https://doi.org/10.1016/j.colsurfb.2005.05.004

14. Xiaowei Wang, Yu Zhang, Tinting Zhang JZ. Effect of dissolved oxygen on elemental sulfur generation in sulfide and nitrate removal process: characterization, pathway, and microbial community analysis. Appl Microbiol Biotechnol. 2016;100(2895–2905):1–11.

15. Moghanloo GMM, Fatehifar E, Saedy S, Aghaeifa Z, Abbasnezhad H. Biological oxidation of hydrogen sulfide in mineral media using a biofilm airlift suspension reactor. Bioresour Technol. 2010;101(21):8330–5. Available from: http://dx.doi.org/10.1016/j.biortech.2010.05.093 https://doi.org/10.1016/j.biortech.2010.05.093

16. Mora Mabel, Fernández Maikel, G\ómez Jos\é Manuel CD, Lafuente Javier GX, David G. Kinetic and stoichiometric characterization of anoxic sulfide oxidation by SO-NR mixed cultures from anoxic biotrickling filters. Appl Microbiol Biotechnol. 2014;99(1):77–87.

17. Alcántara Sergio, Velasco Antonio, Mu-oz Ana, Cid Juan RS, Elías R-F. Hydrogen Sulfide Oxidation by a Microbial Consortium in a Recirculation Reactor System: Sulfur Formation under Oxygen Limitation and Removal of Phenols. Environ Sci Technol. 2004;38(3):918–23. https://doi.org/10.1021/es034527y

18. Nielsen AH, Vollertsen J, Hvitved-Jacobsen T. Kinetics and stoichiometry of aerobic sulfide oxidation in wastewater from sewers-effects of pH and temperature. Water Environ Res. 2006;78(3):275–83. https://doi.org/10.2175/106143005X94367

19. Janssen AJH, Lettinga G, De Keizer A. Removal of hydrogen sulphide from wastewater and waste gases by biological conversion to elemental sulphur. Colloidal and interfacial aspects of biologically produced sulphur particles. Colloids Surfaces A Physicochem Eng Asp. 1999;151(1–2):389–97.

20. Liu H, Wang M, Huang Z, Du H, Tang H. Study on biological control of microbiologically induced corrosion of carbon steel. 2004;55(5):387–91. Available from: http://doi.wiley.com/10.1002/maco.200303749

21. Van Den Bosch PLF, De Graaff M, Fortuny-Picornell M, Van Leerdam RC, Janssen AJH. Inhibition of microbiological sulfide oxidation by methanethiol and dimethyl polysulfides at natron-alkaline conditions. Appl Microbiol Biotechnol. 2009;83(3):579–87. https://doi.org/10.1007/s00253-009-1951-6

22. Abd Aziz Bin Mohd Azoddein, Mani Malam Ahmad RMY, Sulaiman and NMN. A Bioremediation Approach to Mercury Removal in a Shake Flask Culture Using Pseudomonas putida (ATCC 49128) . J Anal Bioanal Tech. 2016;7(3).

23. Mosquera S, González-Jaramillo LM, Orduz S, Villegas-Escobar V. Multiple response optimization of Bacillus subtilis EA-CB0015 culture and identification of antifungal metabolites. Biocatal Agric Biotechnol. 2014;3(4):378–85. Available from: http://linkinghub.elsevier.com/retrieve/pii/S1878818114001157 https://doi.org/10.1016/j.bcab.2014.09.004

24. Mahmood Qaisar, Zheng Ping, Cai Jing, Hayat Yousaf, Hassan Muhammad Jaffar, Wu Dong-lei HB. Sources of sulfide in waste streams and current biotechnologies for its removal. J Zhejiang. 2007;8(7):1126–40. Available from: http://link.springer.com/10.1631/jzus.2007.A1126 https://doi.org/10.1631/jzus.2007.A1126

25. Friedrich Cornelius G., Rother Dagmar, Bardischewsky Frank, Ouentmeier Armin FJ. Oxidation of Reduced Inorganic Sulfur Compounds by Bacteria: Emergence of a Common Mechanism? Appl Environ Microbiol. 2001;67(7):2873–82. https://doi.org/10.1128/AEM.67.7.2873-2882.2001

26. Monod J. The Growth of Bacterial Cultures. Annu Rev Microbiol. 1949;3(1):371–94. https://doi.org/10.1146/annurev.mi.03.100149.002103

27. Okpokwasili GC, Nweke CO. Microbial growth and substrate utilization kinetics. African J Biotechnol. 2005;5(4):305–17.

Article Metrics