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Volume: 2; Issue: 1, Jan-Feb, 2014
Expression and purification of growth hormone from Microminipig (MMP) and its effects on body fat percentage with injection
Shedage Kishor Ashok, Yasushi Kuwabara, Shunnosuke Abe, Eugene Hayato Morita
Anterior pituitary gland of brain releases the growth hormone. It plays an important role in overall body development of human as well as in animal and it is the candidate gene for gain in body weight. Until now, over expression and purification techniques to obtain soluble growth hormones from mini pig and normal pig are studied. In this study, we have developed the more efficient ways to obtain physiologically active recombinant growth hormone of Microminipig. Microminipig is the smallest pig in the world developed by Fuji Nojo Service, Japan, and the weight of this pig is approximately 7.0 kg at the age of 6 months. Recombinant growth hormone was over expressed as a biologically inactive inclusion body. Inclusion body was solubilized with denaturating agents and refolding was carried out by stepwise dialysis technique. To identify the physiological activity of refolded recombinant GH, we injected it to the Microminipigs (75 µg/kg of body weight). We found that the GH injection induces the more efficient acceleration of muscle growth than that for non-injected Microminipig.
Keywords: Growth Hormone, Microminipig, Protein Over expression, Refolding.