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Volume: 6, Issue: 2, March-April, 2018
DOI: 10.7324/JABB.2018.60208

Research Article

Simplified detection of the asymmetric polymerase chain reaction-amplified DNA and its application in the target identification

G Suhasa, Savithri Bhat

Abstract

The nucleic acid amplification methods such as the polymerase chain reaction (PCR) and the loop-mediated isothermal amplification rely on the detection of the amplified products by means of agarose gel electrophoresis. The detection of the amplified target DNA in an asymmetric PCR was simplified by carrying out the probe hybridization, polymerization, and the subsequent measurement of fluorescence of the double-stranded target DNA (dsDNA) using the Qubit® dsDNA BR Assay Kit. This method was validated by detecting the lacZ gene that is present in the pUC 18 plasmid as well as by detecting the CDH13 gene that is present in the human genomic DNA.

Keywords: Polymerase chain reaction, Agarose gel electrophoresis, Asymmetric, Loop-mediated isothermal amplification, Fluorometry.

References

How to cite this article:

Suhasa G, Bhat S. Simplified detection of the asymmetric polymerase chain reaction-amplified DNA and its application in the target identification. J App Biol Biotech. 2018;6(2):50-53. DOI: 10.7324/JABB.2018.60208

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