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Volume: 2; Issue: 1, Jan-Feb, 2014
Repetitive PCR based detection of Genetic Diversity in Xanthomonas axonopodis pv citri Strains
Minhaj Arshiya, Alka Suryawanshi, Digamber More and Mirza Mushtaq Vaseem Baig
Xanthomonas axonopodis pv citri (Xac) is the phytopathogen responsible for citrus canker, one of the most devastating citrus diseases in the world. Fifty six strains of Xanthomonas axonopodis pv citri (Xac) were isolated from different citrus growing areas of Marathwada region of Maharashtra state and twenty representative strains of Xac were selected to study the genetic diversity. Morphological, biochemical and pathogenicity tests were carried out to identify and characterize the strains of bacteria causing citrus canker. Repetitive DNA polymerase chain reaction-based fingerprinting (rep-PCR) using repetitive extragenic palindromic (rep) and enterobacterial repetitive intergenic consensus (ERIC) and BOX primers were used to assess the genetic diversity.Cluster analysis done by combining the banding patterns of REP-PCR, ERIC-PCR and BOX-PCR clearly separated two diverse groups within species of Xanthomonas axonopodis pv citri based on the geographic region of isolation.
Keywords: Citrus canker, Xanthomonas axonopodis pv citri, pathogenicity, genetic diversity, Rep -PCR