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Volume: 2; Issue: 1, Jan-Feb, 2014

Research Article

Repetitive PCR based detection of Genetic Diversity in Xanthomonas axonopodis pv citri Strains

Minhaj Arshiya1, Alka Suryawanshi2, Digamber More1, Mirza Mushtaq Vaseem Baig2

  Author Affiliations


Abstract

Xanthomonas axonopodis pv citri (Xac) is the phytopathogen responsible for citrus canker, one of the most devastating citrus diseases in the world. Fifty six strains of Xanthomonas axonopodis pv citri (Xac) were isolated from different citrus growing areas of Marathwada region of Maharashtra state and twenty representative strains of Xac were selected to study the genetic diversity. Morphological, biochemical and pathogenicity tests were carried out to identify and characterize the strains of bacteria causing citrus canker. Repetitive DNA polymerase chain reaction-based fingerprinting (rep-PCR) using repetitive extragenic palindromic (rep) and enterobacterial repetitive intergenic consensus (ERIC) and BOX primers were used to assess the genetic diversity.Cluster analysis done by combining the banding patterns of REP-PCR, ERIC-PCR and BOX-PCR clearly separated two diverse groups within species of Xanthomonas axonopodis pv citri based on the geographic region of isolation.

Keywords:

Citrus canker, Xanthomonas axonopodis pv citri, pathogenicity, genetic diversity, Rep -PCR



Citation: Minhaj Arshiya, Alka Suryawanshi, Digamber More and Mirza Mushtaq Vaseem Baig. Repetitive PCR based detection of Genetic Diversity in Xanthomonas axonopodis pv citri Strains. J App Biol Biotech. 2014; 2 (01): 017-022.


Copyright: Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited.

References

1. Das, A.K. Citrus canker – A review J. Appl. Hort. 2003;5(1):52-60

2. Anonymous. The Agriculture Diary.,Marathwada Agriculture University Parbhani. 2011.

3. Sujatha B.and Sai Gopal D.V.R. Isolation and characterization of Xanthomonas axonopodis from citrus aurantifolia christm (Swingle). The Bioscan. 2010; 5(3):373-376.

4. Das.A.K and Singh Shyam. PCR based identification of Xanthomonas axonopodispv citri (pathotype A) causing Asiatic citrus bacterial canker disease in India. Indian Phytopathology. 2010. 63(3): 340-342.

5. Louws FJ, Fulbright DW, Stephens CT, de Bruijn FJ .Specific genomic fingerprints of phytopathogenic Xanthomonas and Pseudomonas pathovars and strains generated with repetitive sequences and PCR. Appl Environ Microbiol 1994.60:2286–2295

6. Lee Y. H., Lee S., Lee D.H., Ji S. H., Chang .H. Y., Heu S , Hyun J. W. , Ra D.S. and Park E.W. Differentiation of citrus bacterial canker strains in Korea by host range, rep-PCR fingerprinting and 16S rDNA analysis. Eur J Plant Pathol 2008.121:97–102

7. Cubero, J. and Graham, J.H. Genetic relationship among worldwide strains of Xanthomonas causing canker in citrus species and design of new primers. Appl Environ Microbiol 2002.68, 1257–1264.

8. Arshiya M., Suryawanshi A.R., More, D.R and Baig M.M.V. Genetic and Pathological Diversity among Xanthomonas axonopodis pv citri Strains causing Citrus Canker in Maharashtra, Journal of Pure and Applied Microbiology. 2012; 6(3), 1219-1227.

9. Bradbury J.F.. Genus I. Xanthomonas Dowson.In N. R. Krieg & J. G. Holt Editors Bergey’s Manual of Systematic Bacteriology. Baltimore: Williams & Wilkins, 1984; 1: 63-90.

10. Goszczynska T., Serfontein J.J. & Serfontein S. Introduction to practical phytobacteriology. Switzerland: SAFRINET SDC; 2000

11. Suryawanshi A. R., More S.M. and. Baig M.M.V. Molecular characterization of Xanthomonas citrii pathovars from Marathwada region. Journal of Pure and Applied Microbiology. 2011; 5(2): p.807-812.

12. Lin, H. C., Hsu, S. T., Hwang, A. S., and Tzeng, K. C. Phenotypic and genetic characterization of novel strains of Xanthomonas axonopodis pv citri which induce atypical symptoms on citrus leaves in Taiwan. Plant Pathol. Bull. 2005.14:227-238.

13. Ausbel FH, Brent R, Kingston RE, Moore DD, Seidman JG, Smith JA, Struth K. Current protocols in Molecular Biology. Canada: Wiley. 1994.

14. Trindade, L. C., Lima, M. F., & Ferreira, M. A. S. V. Molecular characterization of Brazilian strains of Xanthomonas campestris pv viticola by rep-PCR fingerprinting. Fitopatologia Brasileira, 2005.30, 46–54.

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