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Volume: 2; Issue: 1, Jan-Feb, 2014

Research Article

Effects of Dietary Lipid Sources on Growth and Survival of Mudfish Heterobranchus longifilis Fingerlings

Reginald Inodu Keremah and Tarila Terimokumo


Effects of four dietary lipid sources (cod liver oil, palm oil, soybean oil, and pork lard) on growth and survival of Heterobranchus longifilis fingerlings were evaluated. Each diet was prepared to contain 40% protein, other nutrients and a lipid source. Diets were tested on triplicate groups of 10 fish per plastic aquarium measuring 45x35x28cm3 with dechlorinated water. Fish were fed daily with 5% of their body weight for 49 days. Best growth (weight gain, specific growth rate and condition) results were obtained with soybean and palm oil diets, the least being cod liver oil. Pork lard diet produced intermediate performance. The feed conversion ratio was superior for soybean oil diet (0.58) compared to others (0.72-0.90). Protein efficiency ratio was similar in all experimental diets, 4.43± 0.74 (palm oil) – 4.98± 0.53 (soybean oil). Survival rates were high for all treatments (81.43±7.05-89.05±3.90%). Economic evaluation and performance of H. longifilis fingerlings suggest soybean oil (34.17/g weight gain) and palm oil (28.75/g. weight gain) diets to be of choice and cheaper to use as potential substitutes or cod liver oil in catfish feeds in this study.

Keywords: Heterobranchus longifilis, diet, lipid, growth, survival.


1. Sarkar SK. Fresh water fish culture. Vol. 1 Delhi-110035: Daya Publishing House; 2002.

2. Hilton JW. The effect of prefasting diet and water temperature on liver glycogen and liver weight on rainbow trout, Salmo gairdneri (Richardson) during fasting. Journal of Fisheries Biology. 1982; 20:69-78.

3. Okoye FC. Eyo AA, Aminu NG. Growth of tilapia Oreochromis niloticus hybrid fingerlings fed lipid-based diets. In: Eyo AA, editor. Proceedings of the 1st National symposium on fish nutrition and fish feed technology, Lagos: Fisheries Society of Nigeria; 2001, p. 52-57.

4. Stickney RR, Hardy RW. Lipid requirements of some warm water species. Aquaculture. 1989; 79:145-156.

5. Dupree HK. Studies on nutrition and feeds of warm water fishes. In: Proceedings of the 1st International Conference on aquaculture nutrition, University of Delaware; 1976.

6. Takeuchi T, Satoh S, Watanabe T. Requirement of Tilapia nilotica for essential fatty acids. Bulletin of the Japanese Society of Scientific Fisheries. 1983; 49: 1127-1134.

7. Watanabe T, Thongrod S, Takeuchi T, Satoh S, Kubota SS, Fujimaki Y, Cho CY. Effect of dietary n-6 and n-3 fatty acids on growth, fatty acid composition and histological changes of white fish Coregonus lavaretus marena. Nippon Suisan Gakkaishi. 1989; 55: 1977-1982.

8. Kanazawa A. Essential fatty acid and lipid requirement of fish. In: Cowey CB, Mackie AM, Bell JG, editors. Nutrition and feeding of fish, London: Academic Press; 1985, p. 281-298.

9. Tacon AGJ. Global trends in aquaculture and compound aqua feed production. In: Tacon AGJ, editor. International aqua feed directory and buyer's guide, Uxbridge, Middlesex: Turret RAI; 2003.

10. Piper GP, McElwain IB, Orme LE, McCraven JP, Fowler LG, Leonard JR. Fish hatchery management. Washington, D.C: United States Department of the Interior, Fish and Wildlife Service; 1982.

11. Oshoke JO, Olukunle OA,Ajayi AI, Dasuki A, Saulawa LA. Haematological changes of Clarias gariepinus juveniles fed different dietary lipid. In: Adeyemo AO, Otobotekere AJT, Keremah RI, Aghoghovwia O, Erhowose E, editors. Proceedings of the 27th Annual Conference of Fisheries Society of Nigeria, Yenagoa: 2012, p. 327-330.

12. Turchini GM, Torstensen BE, Ng WK. Fish oil replacement. In: Finfish nutrition reviews in aquaculture. 2009, p. 10-57.

13. Legendre M, Kerdchuen N, Corraze G, Bergot P. Larval rearing of an African catfish Heterobranchus longifilis (Teleostei, Clariidae): effect of dietary lipid on growth, survival and fatty acid composition of fry. Aquatic Living Resources. 1995; 8:355-363.

14. Ochang SN, Fagbenro OA, Adebayo OT. Growth performance, body composition, haematology and product quality of the African catfish (Clarias gariepinus) fed diets with palm oil. Pakistan Journal of Nutrition. 2007;6:452-459.

15. Lovell T. Nutrition and feeding of fish. New York: An Avi Book, Van Nostrand Reinhold; 1989.

16. Boyd CE. Water quality in warm water fish pond. Opelika, Alabama: Craftmaster Printers Inc; 1979.

17. New MB. Formulated aquaculture feeds in Asia: Some thoughts on comparative economics, industrial potential, problems and research needs in relation to small-scale farmer. In: Bahru J, editor. Report of the workshop on shrimps and finfish development, ASEAN/SF/89/ GEN/11; 1989.

18. Wahua TAT. Applied statistics for scientific studies. Ibadan: Afrika-Link Press; 1999.

19. Sas. Statistical Analysis System Users Guide. SAS/STAT Version. 8th ed. Cary, North Carolina: SAS Institute Inc; 2003.

20. Francis DS, Turchini GM, Jones PL, De Silva SS. Effects of dietary oil source on growth and fillet fatty acid composition of Murray cod, Muccullochella peeli peeli. Aquaculture. 2006; 253:547-556.

21. Stickney RR. Lipids in catfish nutrition. In: Stickney RR, Lovell RT, editors. Nutrition and feeding of Channel catfish, Southern Cooperative Series, USA, Bulletin 218;1977, p. 14-18.

22. Lim PK, Boey BL, Ng WK. Dietary palm oil level affects growth performance, protein retention and tissue vitamin E concentration of African catfish, Clarias gariepinus. Aquaculture. 2001; 202:101-112

23. Maynard LA, Loosli JK, Hintz HF, Warner RG. Animal Nutrition. 7thed. New York: McGraw-Hill; 1979.

24. de Man JM. Principles of food chemistry. Westport, Connecticut: Avi Publishing Company Inc; 1976.

25. Indian Council of Agricultural Research (ICAR). Handbook of fisheries and aquaculture. New Delhi 110 012: Directorate of Information and Publications of Agriculture; 2006.

26. Boyd CE. Water quality management for fish pond culture. New York: Elsevier Scientific Publishing Company; 1982.

How to cite this article:
Reginald Inodu Keremah and Tarila Terimokumo. Effects of Dietary Lipid Sources on Growth and Survival of Mudfish Heterobranchus Longiflis Fingerlings. J App Biol Biotech. 2014; 2 (01): 009-012.



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