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Volume: 1, Issue: 1, May - June, 2013
Study of endophytic Bacillus amyloliquefaciens CC09 and its antifungal cyclic lipopeptides
Cai Xun-Chao, Li Hui, Xue Ya-Rong, Liu Chang-Hong
Endophytic microbes are considered to be one of the most important bioactive compounds pool with a potential application in both pharmaceutical and agrichemical industries since the observation of taxol that could be synthesized by endophytic fungus Taxomyces andreanae. In this study, we reported an endophytic bacterium CC09, which was isolated from healthy Cinnamomum camphora leaves, exhibited a potential to produce antifungal metabolites inhibiting the growth of several phytopathogens such as Glomerella glycines, Rhizoctonia solani and Alternaria alternate. Based on 16S rRNA and rpoB genes analysis and physiological and biochemical assays, the strain CC09 was identified as Bacillus amyloliquefaciens. The antifungal constitutes in the culture filtrate of strain CC09 were predicted to be iturin family based on PCR amplification of specific genes including BmyC, ItuC, ItuD, ItuF and ItuR (encoding Iturin synthethases), FenD and FenE (encoding Fengency synthethases) and SrfC (encoding Surfactin synthethases), and confirmed by high performance liquid chromatography (HPLC). The broad antifungal spectra of the active constitutes in the metabolites indicated that the endophytic bacterium strain CC09 could be used as a potential biocontrol agent to control plant fungal diseases.
Keywords: Endophytic bacterium, Bacillus amyloliquefaciens, iturins, antifungal activity.