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Volume: 4, Issue: 3, May-June, 2016
DOI: 10.7324/JABB.2016.40309

Research Article

Growth, physiological and biochemical responses of Meliaceae species - Azadirachta indica and Melia dubia to elevated CO2 concentrations

S. Janani, P. Priyadharshini, R. S. C. Jayaraj, C. Buvaneswaran, Rekha R. Warrier

  Author Affiliations


Abstract

Response of two important tropical tree species of Meliaceae (Azadirachta indica -neem and Melia dubia - melia) to elevated levels of CO2 (600 and 900 ppm) under simulated temperature and moisture regimes in Automated Open Top Chambers was studied. Growth, biochemical changes, antioxidant property and gas exchange parameters were estimated. The results indicate that A. indica is expected to acclimatize under elevated CO2 concentrations whereas M. dubia was observed to be a species sensitive to elevated CO2 concentrations, affecting the photosynthetic machinery, stomatal conductance and transpiration and a subsequent decrease in carbohydrates, proteins, sugars, amino acids and phenolics. The short-term and long-term responses with respect to stomatal conductance and transpiration rates were higher in neem than melia. Thus, a positive response of neem to increased CO2 concentrations is a good indication for its future establishment in potentially changed climatic conditions.

Keywords:

CO2, Melia dubia, neem, gas exchange, metabolites, growth, native trees, Open Top Chambers.



Citation: Janani S., Priyadharshini P., Jayaraj RSC, Buvaneswaran C., Warrier RR. Growth, physiological and biochemical responses of Meliaceae species-Azadirachta indica and Melia dubia to elevated CO2 concentrations. J App Biol Biotech. 2016; 4 (03): 052-060. DOI: 10.7324/JABB.2016.40309


Copyright: Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited.

References

1. Zhang L, Wu D, Shi H, Zhang C, Zhan X, et al. Effects of Elevated CO2 and N Addition on Growth and N2 Fixation of a Legume Subshrub (Caragana microphyllaLam.) in Temperate Grassland in China. PLoS ONE. 2011; 6(10): e26842.

2. Razzaque, M.A., M.M. Haque, Q.A. Khaliq, A.R.M. Soliman, A. Hamid. Effects of CO2 and nitrogen levels on yield and yield attributes of rice cultivars. Bangl. J. Agric. Res. 2011; 36(2): 213-221.

3. Sharafzadeh, S. and K. Ordookhani. Organic and biofertilizers as a good substitute for inorganic fertilizers in medicinal plants framing. Australian Journal of Basic and Applied Science. 2011; 5(2):1330-1333.

4. Curtis P.S., Drake B.G., Leadly P.W., Arp W.J. and Whigham D.F. Growth and senescence in plant communities exposed to elevated CO2 concentrations on an estuarine marsh. Oecologia. 1989; 78, 20–26.

5. Oberbour, S. F., Strain, B.R. and Fetcher, N. Effect of CO2 enrichment on seedling physiology and growth of two tropical tree species. Physiol. Plant. 1985; 65: 342-356.

6. Norby, R. J. Nodulation and nitrogenase activity in nitrogen-fixing woody plants stimulated by CO2 enrichment of the atmosphere. Physiologia Plantarum. 1987; 71:77-82.

7. O’Neill, B. F., Zangerl, A. R., Delucia, E., And Berenbaum, M. R. Longevity and fecundity of Japanese beetle (Popillia japonica) on foliage grown under elevated carbon dioxide. Environ. Entomol. 2008; 37: 601–607

8. Ainsworth E.A. and Long S.P. What have we learned from 15 years of free-air CO2 enrichment (FACE)? A meta-analytic review of the responses of photosynthesis, canopy. New Phytol., 2005; 165, 351-371.

9. Huang J.G., Bergeron Y., Denneler B., Berninger F. and Tardif J. Response of forest trees to increased atmospheric CO2. Critical Reviews in Plant Sciences. 2007; 26, 265-283.

10. Saxe H., Ellsworth D.S. and Heath J. Tree and forest functioning in an enriched CO2 atmosphere. New Phytol. 1998; 139: 395-436.

11. Curtis P.S. and Wang X.Z. A meta-analysis of elevated CO2 effects on woody plant mass, form, and physiology. Oecologia, 1998; 113, 299-313.

12. Roberntz P. Atmospheric carbon dioxide concentration, nitrogen availability, temperature and the photosynthetic capacity of current-year Norway spruce shoots. Tree Physiol. 2001. 21(12-13):931-40.

13. Wang K.Y., Kellomaki S. and Laitinen K. Effects of needle age, long term temperature and CO2 treatments on the photosynthesis of scots pine. Tree Physiology. 1995; 15: 211-218.

14. Tjoelker M.G., Oleksyn J. and Reich P.B. Seedlings of five boreal tree species differ in acclimation of net photosynthesis to elevated CO2 and temperature. Tree Physiology. 1998; 18: 715-726.

15. Del Pozo, A., Perez, P., Morcuende, R., Alonso, A., Martinez-Carrasco, R. Acclimatory responses of stomatal conductance and photosynthesis to elevated CO2 and temperature in wheat crops grown at varying levels of N supply in a Mediterranean environment. Plant Sci. 2005; 169, 908–916.

16. Zhang, Y., Duan, B., Qiao, Y., Wang, K., Korpelainen, H. and Li, C. Leaf photosynthesis of Betula albosinensis seedlings as affected by elevated CO2 and planting density. Forest Ecology and Management. 2008; 255: 1937–1944.

17. Arnon D.I. Copper enzymes in isolated chloroplasts. Polyphenoloxidase in Beta vulgaris. Plant Physiol., 1949; 24, 1-15.

18. Lowry O.H., Rosebrough N.J, Farr A.L, Randall R.J. Protein measurement with the Folin- phenol reagent. Journal of Biological Chemistry. 1951; 193: 265-275.

19. Hedge J.E. and Hofreiter B.T. Carbohydrate Chemistry, 17 (Eds. Whistler R.L. and Be Miller, J.N.), Academic Press, New York.1962.

20. Miller G.L. Use of Dinitrosalicylic Acid Reagent for Determination of. Reducing Sugar. Analytical Chemistry. 1972; 31:426-428.

21. Moore S. and Stein, W.H. Methods in Enzymol, (Eds. Colowick, S.P. and Kaplan, N.D.) Academic Press, New York. 1948; 468.

22. Malik C.P. and Singh M.B. Plant enzymology and Histo enzymology, Kalyani Publishers, New Delhi. 1980.

23. Lin JY, and Tang CY. Determination of total phenolic and flavonoid contents in selected fruits and vegetables, as well as their stimulatory effect on mouse splenocyte proliferation. Food Chem. 2007; 101:140–147.

24. Amal, A.I.M.; Mokhtar, M.M. and Rasha, M.F. Antitumor and antibacterial activities of [1-(2-ethyl, 6-heptyl) phenol] from Cuminum cyminum seeds. J. Appl. Sci. Res., 2009; 11: 1881-1888.

25. Warrier, RR, Lalitha S, Savitha C. A Modified Assay of Carbonic Anhydrase Activity in Tree Species. BBR-Biochemistry and Biotechnology Reports. 2014; 3(1): 48-55.

26. Gomez KA and Gomez AA. Statistical procedures for agricultural research. 4th Edition. John Wiley and sons, Inc. London, UK. 1984. pp. 13-175.

27. Cortes P, Josep Maria Espelta, Robert Save and Carme Biel. Effects of a nursery CO2 enriched atmosphere on the germination and seedling morphology of two Mediterranean oaks with contrasting leaf habit. New Forests. 2004; 28: 79–88.

28. Tingey D.T., Phillips D.L. and Johnson M.G. Elevated CO2 and conifer roots: effects on growth, life span and turnover. New Phytol. 2000; 147: 87–103.

29. Green, K. and Wright, R. D. Field response of photosynthesis to CO2 enrichment in Ponderosa Pine. Ecology. 1977. 58: 687–692.

30. Wang, Y.P., Rey, A. and Jarvis, P.G. Carbon balance of young birch trees grown in ambient and elevated atmospheric CO2 concentrations. Global Change Biology. 1998; 4: 797–807.

31. Saralabai, V.C., Vivekanandan, M., Babu, R.S. Plant responses to high CO2 concentration in the atmosphere. Photosynthetica. 1997; 33:7–37.

32. Woodward, E.L., Thompson, G.B. and McKee, I.F. The effects of elevated concentrations of carbon dioxide on individual plants, populations, communities and ecosystems. Annals of Botany. 1991; 67: 23–28.

33. Mauney, J. R., Lewin, K. F., Hendrey, G. R. and Kimball, B. A., Growth and yield of cotton expose to free-air CO2 enrichment. Crit. Rev. Plant Sci., 1992; 11: 213–222.

34. Sheshshayee M.S., Krishna Prasad B.T., Natraj K.N., Shankar A.G., Prasad T.G., Uday Kumar M. Ratio of intercellular CO2 concentration to stomatal conductance is a reflection of mesophyll efficiency. Curr. Sci. 1996; 70:672–675.

35. Ogren, W. L. Affixing the O to RUBISCO: Discovering the source of photorespiratory glycolate and its regulation. Photosynth. Res. 2003; 76: 53–63.

36. Palanisamy, K. Interactions of elevated CO2 concentration and drought stress on photosynthesis in Eucalyptus cladocalyx F. Muell. Photosynthetica. 1999; 36: 635-638.

37. Ravichandran, V., Pathmanabhan, G. and Janaguiraman, M.D. Green House Gases Stress. In: Dwivedi, P. and Dwivedi, R.S. (Eds.) Physiology of abiotic stress in plants, Agrobios. 2005; Pp. 35-78.

38. Xia, J.-R. and Gao, K.-S. Impacts of Elevated CO2 Concentration on Biochemical Composition, Carbonic Anhydrase, and Nitrate Reductase Activity of Freshwater Green Algae. Journal of Integrative Plant Biology. 2005; 47: 668–675.

39. Girish Kumar Rasineni, Anirban Guha, Attipalli Ramachandra Reddy. Elevated atmospheric CO2 mitigated photoinhibition in a tropical tree species, Gmelina arborea. J.Photobiol. 2011. 2,24.

40. Strain B.R. and Thomas R.B. Anticipated effects of elevated CO2 and climate change on plants from Mediterranean-type ecosystems utilizing results of studies in other ecosystems. In: Global Change and Mediterranean-Type Ecosystems. Ecological Studies,Vol. 117 (eds J.M. Moreno and W. C. Oechel). 1995; 121–139.

41. Bhatt, R.K. Baig, M.J. Tiwari, H.S. Elevated CO2 influences photosynthetic characteristics of Avena sativa L cultivars. Journal of Environmental Biology. 2010; 31 (5): 813-818.

42. Shahidul Islam, Sharmin Khan and James O. Garner. Elevated Atmospheric CO2 Concentration Enhances Carbohydrate Metabolism in Developing Lycopersicon esculentum Mill. Cultivars. International Journal of Agriculture and Biology. 2006; 8(2): 157-161.

43. Griffin KL, Anderson OR, Gastrich MD, Lewis JD, Lin GH, Schuster W, Seemann JR, Tissue DT, Turnbull MH, Whitehead D.Plant growth in elevated CO2 alters mitochondrial number and chloroplast fine structure. Proceedings of the National Academy of Sciences, USA. 2001; 98:2473-2478.

44. Wiemken, V. and Ineichen, K. Seasonal fluctuations of the levels of soluble carbohydrates in spruce needles exposed to elevated CO2 and nitrogen fertilization and glucose as a potential mediator of acclimation to elevated CO2. Journal of Plant Physiology. 2000; 156: 746-750.

45. Rays Williams., Richard J. Norby and David E. Lincoln. Effects of elevated CO2 and temperature-grown red and sugar maple on gypsy moth performance. Global Change Biology. 2000; 6: 685-695.

46. Rolland F, Moore B, Sheen J. Sugar sensing and signaling in plants. Plant Cell (suppl.).: 2002; 14: S185-S205

47. Wilkins D., Van Oosten J.J., Besford R.T. Effects of elevated CO2 on growth and chloroplast proteins in Prunus avium. Tree Physiol. 1994; 14, 767-779.

48. Lianhong Gu, Paul J. Hanson, W. Mac Post, Dale P. Kaiser, Bai Yang, Ramakrishna Nemani, Stephen G. Pallardy, and Tilden Meyers. The 2007 Eastern US Spring Freeze: Increased Cold Damage in a Warming World. BioScience. 2008; 58(3), 253-262.

49. Gordillo, F.J.L., Niell, F.X. and Figueroa, F.L. Non-photosynthetic enhancement of growth by high CO2 level in the nitrophilic seaweed Ulva rigida C. Agardh (Chlorophyta). Planta. 2001. 213: 64-70.

50. Ingvar Simonsson, Bengt-Harald Jonsson and Sven Lindskog. Phenol, a competitive inhibitor of CO2 hydration catalyzed by carbonic anhydrase. Biochem Biophys Res Commun. 1982; 108(4):1406-1412.

51. Lucina M, F. Rising CO2 levels could reduce protein in crops. Science. 2010; 328:899-903.

52. Rogers, A., Allen, D. J., Davey, P. A., Morgan, P. B., Ainsworth, E. A., Bernacchi, C. J., Cornic, G., Dermody, O., Dohleman, F. G., Heaton, E. A., Mahoney, J., Zhu, X.-G., Delucia, E. H., Ort, D. R. and Long, S. P. Leaf photosynthesis and carbohydrate dynamics of soybeans grown throughout their life-cycle under Free-Air Carbon dioxide Enrichment. Plant, Cell & Environment. 2004; 27: 449–458.

53. Azam S, Hadi N, Khan N.U, Hadi S.M. Prooxidant property of green tea polyphenols epicatechin and epigallocatechin-3-gallate: implications for anticancer properties. Toxicol In Vitro. 2004; 18(5): 55-61.

54. Lindroth R.L. Impacts of Elevated Atmospheric CO2 and O3 on Forests: Phytochemistry, Trophic Interactions, and Ecosystem Dynamics. J Chem Ecol. 2010; 36:2–21.

55. Goncalves, B., Falco, V., Moutinho-Pereira, J., Bacelar, E., Peixoto, F. and Correia, C. Effects of elevated CO2 on grapevine (Vitis vinifera L.): Volatile composition, phenolic content, and in vitro antioxidant activity of red wine. Journal of Agricultural and Food Chemistry. 2009. 57: 265-273.

56. Bridget F. O’Neill, Arthur R. Zangerl, Orla Dermody, Damla D. Bilgin, Clare L. Casteel, Jorge A. Zavala, Evan H. DeLucia, May R. Berenbaum. Impact of Elevated Levels of Atmospheric CO2 and Herbivory on Flavonoids of Soybean (Glycine max Linnaeus). Journal of Chemical Ecology. 2010; 36(1):35-45.

57. Davey PA, Olcer H, Zakhleniuk O, Bernacchi CJ, Calfapietra C, Long SP, Raines CA. Can fast-growing plantation trees escape biochemical down-regulation of photosynthesis when grown throughout their complete production cycle in the open air under elevated carbon dioxide? Plant, Cell and Environment. 2006; 29:1235-1244.

58. Ghasemzadeh A, Jaafar HZE, Rahmat A. Antioxidant activities, total phenolics and flavonoids content in two varieties of Malaysia young ginger (Zingiber officinale Roscoe). 2010. Molecules, 15(6): 4324- 4333.

59. Ghasemzadeh, A. and Jaafar, H.Z.E. Effect of CO2 Enrichment on Some Primary and Secondary Metabolites synthesis in Ginger (Zingiber officinale Roscoe). International Journal of Molecular Science. 2011. 12(2): 1101-1114.

60. Warrier, R.R., Buvaneswaran, C., Priyadharshini, P. and Jayaraj, R.S.C. Growth response of three plantation species of the tropics exposed to elevated CO2 levels. Journal of Forestry Research. 2013; 24: 449–456.

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