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Volume: 3, Issue: 6, Nov-Dec, 2015
DOI: 10.7324/JABB.2015.3610

Research Article

Production and Optimization of Protease by Filamentous Fungus Isolated from Paddy Soil in Thiruvarur District Tamilnadu

Sudarkodi Chandrasekaran1, Sundar Sundaram Pillai Kumaresan1, Murugan Manavalan2

  Author Affiliations


Abstract

This study was under taken to monitor the production of protease enzyme from paddy field soil fungal isolates obtained from Mannargudi, Tamil Nadu. The study also sought to optimize the kinetic parameters of the enzyme with the aim of establishing large scale production of it. Two isolates Aspergillus flavus and Aspergillus niger out of the six were opted as potential candidates for enzyme production following the screening method of plate assay. The results observed were for the maximal production of enzyme by the isolates at various temperatures and pH. Aspergillus flavus and Aspergillus niger showed highest activity in the optimized medium at pH 8.0 and temperature 30 ºC, pH 7.0 and temperature 35 °C, respectively.

Keywords:

Protease, pH, temperature, Aspergillus flavus and Aspergillus niger.



Citation: Sudarkodi C, Sundar SK, Murugan M. Production and Optimization of Protease By Filamentous Fungus Isolated From Paddy Soil In Thiruvarur District Tamilnadu. J App Biol Biotech, 2015; 3 (06): 066-069. DOI: 10.7324/JABB.2015.3610


Copyright: Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited.

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