Home > Past Issue

Volume: 3, Issue: 6, Nov-Dec, 2015
DOI: 10.7324/JABB.2015.3607

Research Article

Impact of Phyllanthus amarus extract on antioxidant enzymes in Drosophila melanogaster

N. Manasa, J. S. Ashadevi

Abstract

Oxidative stress (OS) leads to deleterious effects in organisms. Main cause for the oxidative stress is the generation of free radicals in the cells. Antioxidants have ability to scavenge and neutralize the free radicals. Therefore, present study has undertaken to test the antioxidant potential of Phyllanthus amarus ethanolic extract (PAE) in Drosophila melanogaster through biochemical analysis. OS test has been conducted in the PAE supplemented different age grouped flies by exposing to 20 mM acrylamide (ACR). Antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT) and reduced glutathione (GSH) were measured in different age grouped stressed and non-stressed PAE supplemented flies. OS test results revealed that PAE II treated groups showed significantly increased ACR resistance when compared to PAE I and control groups. All the age grouped PAE supplemented flies in non-stress conditions had significantly high SOD and CAT activities. Highest SOD and CAT activities were observed in PAE II treated 30 and 45 days flies respectively. Under stress conditions, the elevated levels of SOD activities were found in 30 and 45 days and CAT activities in 30, 45 and 60 days aged PAE treated flies. Overall data highlights that there is a dose dependant relationship between the extract concentrations and enzyme activities.

Keywords: Phyllanthus amarus, Drosophila melanogaster, Oxidative stress, Acrylamide, antioxidant enzymes.

References

1. Zheng W, Wang SY. Antioxidant activity and phenolic compounds in selected herbs. Journal of Agriculture and Food Chemistry. 2001; 49: 5165-5170.

2. Beyer W, Imlay J, Fridovich I. Superoxide dismutases. Progress in Nucleic Acid Research and molecular biology. 1991; 40: 221-253.

3. Hasani P, Yasa N, Ghanbari SV, Mohammadirad A, Dehghan G, Abdollahi M. In vivo antioxidant potential of Teucrium polium, as compared to a-tocopherol. Acta Pharmaceutica. 2007; 57: 123-129.

4. Ali SS, Kasoju N, Luthra A, Singh A, Sharanabasava H, Sahu A, Bora U. Indian medicinal herbs as sources of antioxidants. Food Research International. 2008; 41: 1-15.

5. Agati G, Matteini P, Goti A, Tattini M. Chloroplast located flavonoids can scavenge singlet oxygen. New Phytologist. 2007; 174: 77-89.

6. Huda-Fanjan N, Noriham A, Norrakiah AS, Babji AS. Antioxidant activity of plants methanolic extracts containing phenolic compounds. African Journal of Biotechnology. 2009; 8(3): 484-489.

7. Kumaran A, Karunakaran RJ. In vitro antioxidant activities of methanol extracts of five Phyllanthus species from India. LWT-Swiss Society of Food Science and Technology. 2007; 40: 344-352.

8. Kumara KKS, Chethan J, Manasa N, Ashadevi JS, Prakash HS. Bioactive potential of herbaceous Phyllanthus species. International Journal of Pharmacy and Pharmaceutical Sciences, 2012; 4(4): 457-461.

9. Thyagarajan SP, Jayaram S, Gopalakrishnan V, Hari R, Jeyakumar P, Sripathi MS. Herbal medicines for liver diseases in India. Journal of Gastroenterology and Hepatology. 2002; 17: S370-S376.

10. Shetti AA, Sanakal RD, Kaliwal BB. Antidiabetic effect of ethanolic leaf extract of Phyllanthus amarus in alloxan-induced diabetic mice. Asian Journal of Plant Science and Research. 2012; 2(1): 11-15.

11. Manasa N, Ashadevi JS. Herbaceous Phyllanthus species extracts promotes longevity in Drosophila melanogaster. International Journal of Research in Phytochemistry and Pharmacology. 2015; 4(1): 1-9.

12. Ramachandra NB, Ranganath HA. Multifaceted approach to evaluate the relationship among closely related forms of Drosophila. Genome. 1988; 30: 58.

13. Prasad SN, Muralidhara. Neuroprotective effect of geraniol and curcumin in an acrylamide model of neurotoxicity in Drosophila melanogaster: Relevance to neuropathy. Journal of Insect Physiology. 2014; 60: 7\—16.

14. Beauchamp C, Fridovich I. Superoxide dismutase: Improved assays and an assay applicable to acrylamide gels. Analytical Biochemistry. 1971; 44: 276-287.

15. Aebi H. Catalase. In: Bergmeyar HU, editor. Methods in enzymatic analysis. New York: Academic Press; 1984, p. 674-684.

16. Ellman GL. Tissue sulfhydryl groups. Archives of Biochemistry and Biophysics. 1959; 82: 70-77.

17. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with folin phenol reagent. Journal of Biological Chemistry. 1951; 193(1): 265-275.

18. Ara N, Nur H. In vitro antioxidant activity of methanol leaves and flowers extracts of Lippia alba. Research Journal of Medicine and Medical Sciences. 2009; 4: 107-110.

19. Sun Y, Yolitz J, Alberico T, Sun X, Zou S. Lifespan extension by cranberry supplementation partially requires SOD2 and is life stage independent. Experimental Gerontology. 2014; 50: 57-63.

20. Wills NK, Kalariya N, Ramanujam VMS, Lewis JR, Abdollahi SH, Husain A, van Kuijk, FJ. Human retinal cadmium accumulation as a factor in the etiology of age-related macular degeneration. Experimental Eye Research. 2009; 89: 79-87.

21. Minotti G. Metals and membrane lipid damage by oxy-radicals. Annals of the New York Academy of Sciences. 1988; 551: 34-44.

22. Lee KS, Lee BS, Semnani S, Avanesian A, Um CY, Jeon HJ, Seong KM, Yu K, Min KJ, Jafari M. Curcumin extends life span, improves health span, and modulates the expression of age- (associated aging genes) in Drosophila melanogaster. Rejuvenation Research. 2010; 13: 561-570.

23. Zhang Y, Shen T, Liu S, Zhao J, Chen W, Wang H. Effect of Hawthorn on Drosophila melanogaster Antioxidant-Related Gene Expression. Tropical Journal of Pharmaceutical Research. 2014; 13(3): 353-357.

24. Bhattacharya A, Chatterjee A, Ghosal S, Bhattacharya SK. Antioxidant activity of active tannoid principles of Emblica officinalis (amla). Indian Journal of Experimental Biology. 1999; 37: 676-680.

25. Prince PSM, Menon VP. Antioxidant action of Tinospora cordifolia root extract in alloxan diabetic rats. Phytotherapy Research. 2001; 15(3): 213-8.

26. Prince PSM, Menon VP, Pari L. Hypoglycaemic activity of Syzigium cumini seeds: effect on lipid peroxidation in alloxan diabetic rats. Journal of Ethnopharmacology. 1998; 61(1): 1-7.

27. Reddy ACP, Lokesh BR. Studies on spice principles as antioxidants in the inhibition of lipid peroxidation of rat liver microsomes. Molecular and Cellular Biochemistry. 1992; 111: 117-124.

28. Jiang Y. Effects of anthocyanins derived from Xinjiang black mulberry fruit on delaying aging. Wei Sheng Yan Jiu. 2010; 39(4): 451-3.

29. Peng C, Chan HYE, Huang Y, Yu H, Chen ZY. Apple Polyphenols Extend the Mean Lifespan of Drosophila melanogaster. Journal of Agricultural and Food Chemistry. 2011; 59(5): 2097-2106.

How to cite this article:
Manasa N. and Ashadevi J.S. Impact of Phyllanthus amarus extract on antioxidant enzymes in Drosophila melanogaster. J App Biol Biotech, 2015; 3 (06): 043-047. DOI: 10.7324/JABB.2015.3607

ARTICLE TOOLS

ARTICLE STATISTICS

  • Viewed: 128
  • Downloaded: 123

  Search by Author


      

  Search in Pubmed


   Search in Google Scholar