Home >Archive

Volume: 3, Issue: 6, Nov-Dec, 2015
DOI: 10.7324/JABB.2015.3606

Research Article

Insight into interplay of some molecular chaperones in a hill stream teleost, Barilius bendelisis under wide seasonal and diurnal thermal fluctuations

Saurabh Dewan, Yashpal Singh, JP Bhatt

  Author Affiliations


Barilius bendelisis, one of the principal hill stream fish routinely experiences up to 5-6 °C of diurnal and 18-20°C of annual habitat temperature variations. The role of molecular chaperones like HSPs in enabling adjustment to these two temporally distinct patterns of thermal stress is still less explored. 2-D Gel Electrophoretic brain profile of different laboratory acclimatized populations according to seasonal and daily temperature variations, showed induction of different classes of Heat Shock proteins with different endogenous levels and variation in onset temperatures as well. Low molecular weight HSPs appear to play an important role in fluctuating temperatures while the larger weight chaperones (HSP70, 90) in chronic high temperatures. Further, individuals acclimatized to Summer-like temperature exhibited higher endogenous levels of HSPs as compared to winter-acclimatized individuals. The HSP induction temperature in winter-acclimatized fishes was around 20°C whereas the first appearance of HSPs in summer caught as well as ‘winter caught-summer acclimatized’ individuals was 30-32°C; an overall shift of 10-12°C that could well occur on a seasonal basis. These findings provide first molecular insight into survival strategy of a major hill stream teleost, Barilius bendelisis and suggest that variation in constitutive expression of HSPs plays significant role in imparting tolerance against thermal stress.

Keywords: HSPs, seasonal variation, thermal stress, 2D gel electrophoresis, cyprinid.

Citation: Dewan S, Singh Y and Bhatt JP. Insight into interplay of some molecular chaperones in a hill stream teleost, Barilius bendelisis under wide seasonal and diurnal thermal fluctuations. J App Biol Biotech, 2015; 3 (06): 034-042. DOI: 10.7324/JABB.2015.3606

Copyright: Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited.


1. Torgersen CE, Price DM, Li HW, McIntosh BA. Multiscale thermal refuge and stream habitat associations of Chinook salmon in North-eastern Oregon. Ecological Applications. 1999; 9:301-319.

2. Feder ME, Hofmann GE. Heat-shock proteins, molecular chaperones, and the stress response: evolutionary and ecological physiology. Annual Review of Physiology. 1999; 61:243-282.

3. Fujita J. Cold shock response in mammalian cells. Journal of Molecular Microbiology and Biotechnology. 1999; 1:243-255.

4. Morimoto RI, Jurivish DA, Kroeger PE, Mathur SK, Murphy SP, Nakai A, Sarge K, Abravaya K, Sistonen LT. Regulation of heat shock gene transcription by a family of heat shock factors. In: Morimoto RI, Tissieres A, Georgopoulos C, editors. The biology of heat shock proteins and molecular chaperones, New York: Cold Spring Harbor Laboratory Press; 1994, p. 417-455.

5. Kiiltz D, Burg M. Evolution of osmotic stress signalling via MAP kinase cascades. Journal of Experimental Biology. 1998; 201:3015-3021.

6. Owen ME, Hofmann GE. Resetting the thermostat: Changes in HSP induction temperatures in Mytilus trossulus. American Zoologist. 1998; 38:48A.

7. Purohit GK, Mahanty A, Suar M, Sharma AP, Mohanty BP, Mohanty S. Investigating hsp Gene Expression in Liver of Channa striatus under Heat Stress for Understanding the Upper Thermal Acclimation. BioMed Research International. 2014 June 9; 381719.doi: org/10.1155/2014/381719.

8. Mohanty BP, Bhattacharjee S, Mondal K, Das MK. HSP70 expression profiles in white muscles of riverine catfish Rita rita show promise as biomarker for pollution monitoring in tropical rivers. National Academy Science Letters. 2010; 33(5&6):177-182.

9. Basu N, Todgham AE, Ackerman PA, Bibeau MR, Nakano K, Schulte PM, Iwama GK. Heat shock protein genes and their functional significance in fish. Gene. 2002; 295:173-183.

10. Sorensen J, Loeschcke V. Decreased heat-shock resistance and down-regulation of HSP70 expression with increasing age in adult Drosophila melanagaster. Functional Ecology. 2002; 16:379-384.

11. Goswami M, Hariprasad G, Dubey A, Kumar R, Nagpure NS, Srinivasan A, Singh TP, Lakra WS. Proteomics Analysis of Liver Tissue of Labeo rohita. Current Proteomics. 2015; 12:56-62.

12. Abbaraju NV, Boutaghou MN, Townley IK, Zhang Q, Wang G, Cole RB, Rees BB. Analysis of Tissue Proteomes of the Gulf Killifish, Fundulus grandis by 2D Electrophoresis and MALDI-TOF/TOF Mass Spectrometry. Integrative and Comparative Biology. 2012; 52:626-635.

13. Wang M, Chan LL, Si M, Hong H and Wang D. Proteomic analysis of hepatic tissue of zebrafish (Danio rerio) experimentally exposed to chronic microcystin-LR. Toxicological Science. 2009; 113(1):60-69.

14. Wulff T. Long term anoxia in rainbow trout investigated by 2-DE and MS/MS. Proteomics. 2008; 8:1009-1018.

15. Mortz E, Krogh TN, Vorum H, Görg A. Improved silver staining protocols for high sensitivity protein identification using matrix-assisted laser desorption/ionization-time of flight analysis. Proteomics. 2001; 1:1359-1363.

16. Norris CE, Brown MA, Hickey E, Weber LA, Hightower LE. Low-molecular-weight heat shock proteins in a desert fish (Poeciliopsis lucida): Homologs of human HSP27 and Xenopus HSP30. Molecular Biology and Evolution. 1997; 14:1050-1061.

17. Hightower LE, Norris CE, Di Iorio PJ, Fielding E. Heat shock responses of closely related species of tropical and desert fish. American Zoologist. 1999; 39:877-888.

18. Lowe CH, Heath WG. Behavioral and physiological responses to temperature in the desert pupfish Cyprinodon macularius. Physiological Zoology. 1969; 42:53-59.

19. Feldmeth CR, Stone EA, Brown JH. An increased scope for thermal tolerance upon acclimating pupfish (Cyprinodon) to cycling temperatures. Journal of Comparative Physiology. 1974; 89:39-44.

20. Otto RG. The effects of acclimation to cyclic thermal regimes on heat tolerance of the western mosquito fish. Transactions of American Fisheries Society. 1974; 331-335.

21. Shrode JB, Gerking SD. Effects of constant and fluctuating temperatures on reproductive performance of a desert pupfish, Cyprinodon nevadensis nevadensis. Physiological Zoology. 1977; 50:1-10.

22. Woiwode JG, Adelman IR. Effects of starvation, oscillating temperatures, and photoperiod on the critical thermal maximum of hybrid striped x white bass. Journal of Thermal Biology. 1992; 17:271-275.

23. Heath AG, Turner BJ, Davis WP. Temperature preferences and tolerances of three fish species inhabiting hyperthermal ponds on mangrove islands. Hydrobiologia. 1993; 259:47-55.

24. Podrabsky JE, Somero GN. Changes in gene expression associated with acclimation to constant temperatures and fluctuating daily temperatures in an annual killifish Austrofundulus limnaeus, The Journal of Experimental Biology. 2004; 207:2237-2254.

25. Craig EA, Gross LA. Is HSP70 the cellular thermometer? Trends in Biochemical Sciences. 1991; 16:135-140.

26. Dietz TJ, Somero GN. The threshold induction temperature of the 90-kDa heat shock protein is subject to acclimatization in eurythermal goby fishes (Genus Gillichthys). Proceedings of National Academy of Science USA. 1992; 89:3389-3393.

27. Fader SC, Yu Z, Spotila JR. Seasonal variation in heat shock proteins (HSP70) in stream fish under natural conditions. Journal of Thermal Biology. 1994; 19:335-341.

28. Hofmann GE, Somero GN. Evidence for protein damage at environmental temperatures: Seasonal changes in levels of ubiquitin conjugates and HSP70 in the intertidal mussel, Mytilus trossulus. Journal of Experimental Biology. 1995; 198:1509-1518.

29. Roberts DA, Hofmann GE, Somero GN. Heat-shock protein expression in Mytilus californianus: Acclimatization (seasonal and tidal height comparisons) and acclimation effects. The Biological Bulletin. 1997; 192:309-320.

30. Buckley BA, Owen ME, Hofmann GE. Adjusting the thermostat: the threshold induction temperature for the heat-shock response in intertidal mussels (Genus Mytilus) changes as a function of thermal history. Journal of Experimental Biology. 2001; 204:3571-3579.

Article Metrics