Home > Past Issue
Volume: 3, Issue: 6, Nov-Dec, 2015
Species relationships in Chenopodium quinoa and Chenopodium album on the basis of morphology and SDS-PAGE profiles of soluble seed proteins
Rajkumari Jashmi Devi, Nikhil K Chrungoo
Nineteen accessions of Chenopodium, comprising of 11 accessions of C. quinoa and 8 accessions of C. album were evaluated. Morphological variations were observed in leaf colour, shape and margins, type of pollen grains, seed colour, seed coat texture and morphology of seed edges amongst the accessions. SDS-PAGE profiling of soluble seed proteins revealed 22 bands ranging in molecular mass from 26-68 kDa. While 6 protein bands were monomorphic, 16 protein bands were polymorphic. One band representing protein with molecular mass of 35.76 kDa was detected only in the accession IC-341704 of C. album. Five exotic accessions viz. EC-507744, EC-507742, EC-507738, EC-5077391 and EC-507741, belonging to C. quinoa, showed the presence of a duplex of 33.55 and 29.59 kDa bands which was not detected in any other accession. The dendrogram generated from the scoring profiles of SDS-PAGE of soluble seed proteins segregated the accession as per their taxonomic classification into C. quinoa and C. album. While the accessions belonging to C. album showed a high degree of morphological heterogeneity, the SDS-PAGE profiles of seed proteins indicated low level of variations within the accessions of each species. Further, the accessions IC-411824 and IC-411825 showed more closeness with C. quinoa than C. album.
Keywords: Chenopodium quinoa, C. album, SDS-PAGE, soluble seed protein.